The distribution of Bifidobacterium in stomach and intestines of ten healthy rabbits were studied by microecological methods. The results showed that Bifidobacterium exist in stomach, small intestine and large intestine, the amount hightest in large intestine, second in jejunum and no finnd in duodenum. Being isolated, purified and biologically identified, the bacteria was identified as Bifidobacterium cuniculi, whose other biological characteristics also was studied.