质量细胞仪
染色
抗体
细胞仪
流式细胞术
化学
一级和二级抗体
病理
分子生物学
生物医学工程
生物
医学
表型
免疫学
生物化学
基因
作者
Qing Chang,Olga Ornatsky,David W. Hedley
摘要
This unit describes protocols for labeling tissue sections using combinations of metal-tagged antibodies and an iridium-containing DNA intercalator for analysis by imaging mass cytometry. Imaging mass cytometry (IMC) allows the labeling of up to 40 individual markers simultaneously using antibody cocktails. We discuss labeling of both cryostat sections and sections from formalin-fixed, paraffin-embedded (FFPE) tissue blocks. The protocols are similar to those used for optical microscopy techniques, while allowing much higher complexity of analysis. © 2017 by John Wiley & Sons, Inc.
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