七鳃鳗
多克隆抗体
免疫原
单克隆抗体
抗体
生物
细胞生物学
效应器
分子生物学
重组DNA
生物化学
免疫学
基因
渔业
作者
Meng Nie,Bo Feng,Chang Liu,Yijun Tu,Xiaoni Chen,Fenfang Wu
标识
DOI:10.1016/j.pep.2021.106008
摘要
In the most primitive jawless vertebrate lamprey, the complement-dependent cytotoxicity regulated by variable lymphocyte receptors (VLRs) plays an important role in the adaptive immunity. Our previous studies have shown that the lamprey pore-forming protein (LPFP) acted as the terminal effector of VLR to lyse and kill the target cells. Here, the recombinant GST-LPFP protein was expressed and purified in prokaryotic expression system, and then used as the immunogen to produce mouse monoclonal antibody and rabbit polyclonal antibody. With these antibodies, we proved that LPFP existed as homodimers in the lamprey serum, and could be recruited to the membrane of target cells after stimulation. In conclusion, the antibodies we produced could specifically recognize the LPFP protein, which could be the useful tools to further study the pore-forming mechanism of LPFP.
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