生发中心
生物
BCL6公司
分子生物学
CD40
卵泡期
B细胞
过继性细胞移植
白细胞介素21
抗原
细胞生物学
免疫学
T细胞
抗体
细胞毒性T细胞
体外
遗传学
免疫系统
CD8型
作者
Chen-Hao Yeh,Joel Finney,Takaharu Okada,Tomohiro Kurosaki,Garnett Kelsoe
出处
期刊:Immunity
[Elsevier]
日期:2022-02-01
卷期号:55 (2): 272-289.e7
被引量:24
标识
DOI:10.1016/j.immuni.2021.12.015
摘要
T follicular helper (Tfh) cells are defined by a Bcl6+CXCR5hiPD-1hi phenotype, but only a minor fraction of these reside in germinal centers (GCs). Here, we examined whether GC-resident and -nonresident Tfh cells share a common physiology and function. Fluorescently labeled, GC-resident Tfh cells in different mouse models were distinguished by low expression of CD90. CD90neg/lo GCTfh cells required antigen-specific, MHCII+ B cells to develop and stopped proliferating soon after differentiation. In contrast, nonresident, CD90hi Tfh (GCTfh-like) cells developed normally in the absence of MHCII+ B cells and proliferated continuously during primary responses. The TCR repertoires of both Tfh subsets overlapped initially but later diverged in association with dendritic cell-dependent proliferation of CD90hi GCTfh-like cells, suggestive of TCR-dependency seen also in TCR-transgenic adoptive transfer experiments. Furthermore, the transcriptomes of CD90neg/lo and CD90hi GCTfh-like cells were enriched in different functional pathways. Thus, GC-resident and nonresident Tfh cells have distinct developmental requirements and activities, implying distinct functions.
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