Biopharmaceutics plays an important role in generic drug development. Understanding the regulatory requirements is paramount while developing the generic formulation for various market. In this review article an attempt was made to compare major regulatory biopharmaceutics guidelines for BCS based biowaiver, Dissolution profiles comparison, IVIVC and PBPK/PBBM modeling. Overall this publication provides comprehensive overview of all biopharmaceutics related guidance’s at one place for readers and as well as industry.