Nudifloids W‐Y ,Three New 3,4‐Seco‐Labdane Diterpenoids with Anti‐inflammatory Activity from Callicarpa Nudiflora

Three new seco-labdane diterpenoids, named nudifloids W-Y (1-3), along with twelve known diterpenoids (4-15) and one sesquiterpenoid (16) were isolated from the stems and leaves of Callicarpa nudiflora. The chemical structures of compounds 1-3 were determined by 1D and 2D NMR, HR-ESI-MS, and electronic circular dichroism (ECD). The chemical structures of the remaining thirteen known compounds were determined by comparing their spectral data with those reported in the literature. All compounds were screened for anti-inflammatory activity by analyzing the detection of lactate dehydrogenase (LDH) release in J771A.1 cells, among which compound 3 showed moderate activity with an IC50 value of 2.10 ± 0.21 μM demonstrating that compound 3 inhibited lipopolysaccharide and nigericin-induced pyroptosis in J774A.1 cells in a dose-dependent manner.