Preparation of Polydeoxyribonucleotide Nanoliposomes and their Applicability to Cosmetic Formulations

Introduction: In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated using the Franz diffusion cell method. Method: In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated using the Franz diffusion cell method. Results: Results indicated that the cumulative skin permeation of PDRN-NL over 24 hours was 1.22 times higher than that of free PDRN, with skin retention of PDRN-NL being 1.40 times greater. The in vitro release studies demonstrated that liposomal encapsulation not only enhanced permeability but also provided a sustained-release effect and improved stability of PDRN. Conclusion: Overall, the properties of liposome-encapsulated PDRN were significantly enhanced, presenting a novel solution for the incorporation of PDRN in the development of skincare products.