3D生物打印
类有机物
细胞外基质
透明质酸
丝素
生物医学工程
材料科学
乳腺癌
组织工程
癌症研究
化学
细胞生物学
癌症
生物
医学
解剖
丝绸
复合材料
遗传学
作者
Wen Shi,Sameer Mirza,Mitchell Kuss,Bo Liu,Andrew Hartin,Shibiao Wan,Yunfan Kong,Bhopal Mohapatra,M. Krishnan,Hamid Band,Vimla Band,Bin Duan
标识
DOI:10.1002/adhm.202300905
摘要
Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes of tumor surrounding cells to recapitulate key tumor hallmarks. Collagen is a major extracellular matrix protein for solid tumors, but low viscosity of collagen solution has made 3D bioprinted cancer models challenging. This work produces embedded, bioprinted breast cancer cells and tumor organoid models using low-concentration collagen I based bioinks. The biocompatible and physically crosslinked silk fibroin hydrogel is used to generate the support bath for the embedded 3D printing. The composition of the collagen I based bioink is optimized with a thermoresponsive hyaluronic acid-based polymer to maintain the phenotypes of both the noninvasive epithelial and invasive breast cancer cells, as well as cancer-associated fibroblasts. Mouse breast tumor organoids are bioprinted using optimized collagen bioink to mimic in vivo tumor morphology. A vascularized tumor model is also created using a similar strategy, with significantly enhanced vasculature formation under hypoxia. This study shows the great potential of embedded bioprinted breast tumor models utilizing a low-concentration collagen-based bioink for advancing the understanding of tumor cell biology and facilitating drug discovery research.
科研通智能强力驱动
Strongly Powered by AbleSci AI