Bacteriochlorophyll reveals estuarine hypoxia and anoxygenic photosynthetic carbon fixation

Abstract Bacteriochlorophylls (Bchls) are photosynthetic pigments produced by aerobic anoxygenic photosynthetic bacteria and anoxygenic photosynthetic bacteria. However, the sources, degradation, and influence of redox conditions in marine environments remain unclear. This study investigated the distribution of Bchls in the Changjiang Estuary to evaluate their origins, degradation, and environmental response. Results showed that total bacteriochlorophylls a (T Bchl a ), including bacteriochlorophyll a (Bchl a ) and its degradation product bacteriopheophytin a (Bphe a ), were higher in surface waters than in bottom waters in the inner estuary, where microbial evidence ( Sphingomonadaceae ) identified aerobic anoxygenic photosynthetic bacteria as the dominant source of T Bchl a . In contrast, the outer estuary exhibited elevated T Bchl a in bottom waters, with purple sulfur bacteria ( Chromatiaceae and Ectothiorhodospiraceae ) as the primary contributors. Significant negative correlations between Bphe a concentrations in bottom seawaters and surface sediments and the dissolved oxygen levels in bottom waters, along with a positive correlation between Bphe a in the surface sediments and the salinity difference between bottom and surface seawaters (Δ S ), suggest that Bphe a may serve as a useful indicator of hypoxia and water stratification. The contribution of anoxygenic photosynthetic bacteria to carbon fixation is expected to increase notably, potentially reaching 16% of phytoplankton carbon fixation after 100 years under expanding hypoxia. These findings establish Bchls as reliable indicators of hypoxia and water stratification and provide a framework for reevaluating carbon fixation in global hypoxic coastal systems.