Human breast cancer patient tissue dissociation for single-cell RNA sequencing v1

Efficient and gentle dissociation of solid tumors is essential for preserving cell viability and transcriptomic integrity in single-cell RNA sequencing workflows. This protocol outlines an optimized approach for processing fresh human breast cancer tissue, designed to minimize cell stress while maximizing viable cell recovery. By combining enzymatic digestion with mechanical dissociation, the method yields high-quality single-cell suspensions with consistently high viability and strong transcript capture, enabling reliable downstream analysis of the tumor microenvironment at single-cell resolution.