解码方法
炸薯条
电化学
纳米技术
计算机科学
嵌入式系统
计算机硬件
化学
材料科学
电极
电信
物理化学
作者
Yichen Liu,Yidan Tang,Yungang Bao,Kun Cai,Baiyang Lu,Rujian Zhao,C. X. Yu,Yan Du,Bingling Li
标识
DOI:10.1002/ange.202400340
摘要
In order to realize portable pathogen diagnostics with easier quantitation, digitization and integration, we develop a ready‐to‐use electrochemical sensing strategy (Iso‐E‐Codelock) for real‐time detection of isothermal nucleic acid amplification. Bridged by a branch DNA as codelock, the isothermal amplicon is transduced into increased current of an electrochemical probe, holding multiple advantages of high sensitivity, high selectivity, signal‐on response, “zero” background and one‐pot operation. Through a self‐designed portable instrument (BioAlex PHE‐T), the detection can implement on a multichannel microchip and output real‐time amplification curves just like an expensive commercial PCR machine. The microchip is a rebuilding‐free and disposable component. The branch codelock probe can be customized for different targets and designs. Such high performance and flexibility have been demonstrated utilizing four virus (SARS‐CoV‐2, African swine fever, Flu A and Flu B) genes as targets, and two branch (3‐way and 4‐way) DNAs as codelock probes.
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