Inhibition of HBsAg and HBeAg Expression by ASODN of Various Regions of the HBV Genome

Objective To study the effect of antisense phosphorothioate oligodeoxynucleotides (S-as ODN) of various regions of the HBV genome on the expression of HBsAg and HBeAg in the HepG22215 cell line. Method The expression levels of HBsAg and HBeAg in the HepG22215 cell line were determined by ELISA. According to gene structure of HBV, 5 S-as ODNs were designed and synthesized. The effect of different ASODNs on the expression of HBsAg and HBeAg was analyzed. Results This confirmed the characteristics of HepG22215 which could produce HBsAg and HBeAg. All 5 S-as ODNs could specifically inhibit the production of HBsAg and HBeAg in HepG22215 cells. A combination of drug and ASODN could enhance the inhibition. Conclusion These results suggest that S-as ODN may be a potential new therapy for anti-HBV. The inhibition showed sequence-specific, dose-dependent and drug-cooperative effects.