The hypothesis of nanofiltration using disused reverse osmosis membrane elements was introduced. This disused reverse osmosis membrane element was regenerated by chemical cleaning and surface oxidation. By property analysis of this treated membrane, it is concluded that under low operating pressure (0.3~0.7 MPa), regenerated membrane could have similar performances as nanofiltration membrane in rejection of univalent ions, bivalent ions and macromolecule organic matters. Applications of this regenerated membrane were reviewed.