In a clinical study the automated donor plasmapheresis devices Autopheresis-C A 100, Plasmapur-Monitor, and Ultralite-PCS were tested. Including also manual plasmapheresis, we analyzed the efficiency of donor plasmapheresis, the reliability and breakdown susceptibility of the methods, the quality of the collected fresh plasma, and the suitability of the different machine plasmapheresis devices for routine application, using a total of 2,372 donor plasmaphereses. We also studied the behavior of some individual laboratory parameters due to plasmapheresis with Plasmapur-Monitor. The data allow us to recommend machine plasmapheresis for routine operation (Ultralite-PCS greater than Autopheresis-C A 100 greater than Plasmapur-Monitor).