Development of Cell Image Based on Second Harmonic Generation

Second harmonic generation(SHG) imaging has the advantages of large penetration depth,high spatial and temporal resolution and low photodamage.Because of its sensitivity to the symmetry change of the microstructure,SHG imaging technique becomes to be one of the hot topics in bio-imaging research.The principle and the applications of SHG imaging are demonstrated in this paper,and the focus of SHG technique is on the development of intracellular SHG imaging that various labels,including voltage sensitive dyes,chiral molecule and metal nanoparticles are used.