Study on The Histologic Change of Rat Ovary Tissue After Culturing in Vitro

Objective:To search for the most adequate duration and conditions of tissue culture to reduce the immunogenicity and therefore raise survival rate of the cultivated tissue after transplantation.Methods:The changes of morphology and enzymatic activity of the cultivated ovary tissue were observed before and after cultivating for 1d、2d and 3d respectively.Results:Ovarian enzyme activity appeared significant differences as compared with unculfured ovary within two day's culture.Conclusion:Under the same cultural conditions,the most appropriate cultural duration for the rat's ovary tissue is within two days.