Research of the relationship between the length of spacer and probe and the repetition of oligonucleotide chip

Research of th e relationship between the length of spacer and probe and the repetition of oligon ucleotide chip.Design12probes with different spacers,then let them hybridiz e with labeled target of749bp.Repeat it three times,scan and quantify the i mage with Quantarray software.The coefficients of variation tend to debase whil e the length of probe extends.The probes of15mer with different spacers have poor and unstable hybridization signal and the repetition is relatively poor.T he probes of20mer?25mer and30mer have relatively well repetition.The coe ffi-cients of variation are the least when the spacer is15.The25mer or30 mer probe with poly(dT)15as spacer has the best repetition.