原生质体
转化(遗传学)
核酸
脂质体
DNA
生物
卡那霉素
植物细胞
膜
基因传递
细胞生物学
基因
生物物理学
生物化学
化学
遗传增强
标识
DOI:10.1111/j.1399-3054.1990.tb05882.x
摘要
Liposomes spontaneously interact with the plasma membrane of protoplasts. The delivery of their content to the protoplast, however, has to be induced by physical or chemical treatments. Three major approaches have been used to study and optimize the delivery of RNA or DNA molecules: viral infection, transient gene expression and stable transformation. Conclusive evidence for the delivery of nuclei acids to the treated protoplasts has been obtained. Stable transformants expressing kanamycin resistance have been further characterized by hybridization techniques and progeney analysis. The potential and limitations of this approach for the transformation of higher plants is discussed.
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