Tunnel-current based single-molecule identification of DNA/RNA oligmer by using nano-MCBJ

We have been proposed a tunneling-current identification methodology of a DNA/RNA sequence, which is based on sequentially reading the tunneling-current across individual single-nucleotides in the sequence In this report, we determined the single-molecule electrical conductance for all kinds of deoxyribonucleotide, and ribonucleotides at the single-nucleotide level, by using gap-tunable gold nano-electrode. Based on the electrical conductivity, we electrically identified the base-type in oligonucleotide, and read the fragment of sample nucleotide passing through the sensing electrode. This method could be one of the promising whole-genome sequencing strategies.