烟草花叶病毒
运动蛋白
生物
烟草
烟草
突变体
番茄斑萎病毒属
病毒学
烟草病毒
马铃薯X病毒
打开阅读框
病毒
氨基酸
黄瓜花叶病毒
植物病毒
融合蛋白
肽序列
生物化学
核糖核酸
基因
重组DNA
番茄斑点萎蔫病毒
外壳蛋白
作者
Yongqiang Zhang,Chao Zhang,Weimin Liu
出处
期刊:Virus Research
[Elsevier]
日期:2012-01-01
卷期号:163 (1): 246-253
被引量:9
标识
DOI:10.1016/j.virusres.2011.10.006
摘要
To investigate the potential role(s) of the nucleocapsid (N) protein of Tomato spotted wilt virus (TSWV), the open reading frame for the N protein was expressed from a Tobacco mosaic virus (TMV)-based vector encoding only the TMV replicase proteins. In the absence of other TSWV-encoded proteins, the transiently expressed N protein facilitated long-distance movement of the TMV-based hybrids in transgenic Nicotiana benthamiana [NB-MP(+)] expressing movement protein of TMV, thus providing the functional demonstration of the N protein in long-distance RNA movement. Removal of the N-terminal 39 amino acids (N-NΔ39), the C-terminal 26 amino acids (N-CΔ26) or both of them (N-NΔ39CΔ26) abolished the long-distance movement function, indicating the essential role of both N- and C-terminus. In contrast, alanine substitution of the phenylalanines at positions 242 and 246 (N242/262A), two crucial amino acids for homotypic interaction of the N protein, had little effect, suggesting that the N protein could function in long-distance movement in the form of monomers. In addition, both the wild type N and the alanine mutant N242/262A hardly induced local symptoms in NB-MP(+) plants and TMV-MP transgenic N. tabacum cv. Xanthi. The deletion mutants N-NΔ39, N-CΔ26 and N-NΔ39CΔ26, however, induced apparent symptoms of necrotic ringspots, necrosis or chlorotic spots in all inoculated leaves. On the basis of these findings, the potential role of N during the TSWV infection was discussed. To our knowledge, this is the first report that the N protein of an enveloped plant virus functioned in long-distance movement.
科研通智能强力驱动
Strongly Powered by AbleSci AI