脊髓
核糖核酸
生物
RNA提取
肠道病毒
逆转录酶
冰冻切片程序
分子生物学
病毒学
病毒
病理
医学
基因
生物化学
神经科学
作者
Frédérik Beaulieux,M. Berger,Rémy Tcheng,P Giraud,Bruno Lina
标识
DOI:10.1016/s0166-0934(02)00209-4
摘要
Methods for detecting enterovirus RNA in both paraffin-embedded, formalin-fixed and frozen spinal cord sections from amyotrophic lateral sclerosis (ALS) patients were established. A proteinase K digestion following the deparaffinization procedure was required for the fixed spinal cord sections, whereas only one step of crushing in phosphate buffered saline was necessary for the frozen samples prior to the extraction of the RNA. With an optimized reverse transcription and PCR procedure, enterovirus RNA could be detected from frozen and fixed archival spinal cord samples.
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