纳米复合材料
石墨烯
电催化剂
电极
电化学
六氟磷酸盐
无机化学
工作电极
电子转移
参比电极
化学修饰电极
化学
硫化物
离子液体
硫化铜
材料科学
铜
纳米技术
催化作用
光化学
有机化学
物理化学
作者
Fan Shi,Weizhe Zheng,Wencheng Wang,Fei Hou,Bing‐Xin Lei,Zhenfan Sun,Wei Sun
标识
DOI:10.1016/j.bios.2014.08.064
摘要
In this paper a graphene (GR) and copper sulfide (CuS) nanocomposite was synthesized by hydrothermal method and used for the electrode modification with a N-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. Hemoglobin (Hb) was immobilized on the modified electrode to get a biocompatible sensing platform. UV-vis absorption spectroscopic results confirmed that Hb retained its native secondary structure in the composite. Direct electron transfer of Hb incorporated into the nanocomposite was investigated with a pair of well-defined redox waves appeared on cyclic voltammogram, indicating the realization of direct electrochemistry of Hb on the modified electrode. The results can be ascribed to the presence of GR-CuS nanocomposite on the electrode surface that facilitates the electron transfer rate between the electroactive center of Hb and the electrode. The Hb modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid in the concentration range from 3.0 to 64.0 mmol L(-1) with the detection limit of 0.20 mmol L(-1) (3σ). The fabricated biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability.
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