小RNA
细胞生物学
生物
计算生物学
核糖核酸
基因表达调控
基因表达
转录后调控
基因沉默
基因
细胞
化学
转录因子
德罗沙
转录组
作者
Wendao Liu,Noam Shomron
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2243: 339-354
被引量:1
标识
DOI:10.1007/978-1-0716-1103-6_18
摘要
MicroRNAs (miRNAs) regulate gene expression by binding to mRNAs. Consequently, they reduce target gene expression levels and expression variability, also known as Single-cell RNA sequencing (scRNA-seq) technology has been used to study miRNA and mRNA expression in single cells, and has demonstrated its strength in quantifying cell-to-cell variation. Here we describe how to investigate miRNA regulation using data with both mRNA and miRNA expression in single cell format. We show that miRNAs reduce the expression levels and also expression noise of target genes in single cells. Finally, we also discuss potential improvements in experimental design and computational analysis of scRNA-seq in order to reduce or partition the technical noise.
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