生物
上皮
气道
呼吸上皮
细胞
流式细胞术
免疫学
细胞生物学
核糖核酸
电池类型
基因
医学
遗传学
外科
作者
Luke R. Bonser,Kyung S. Koh,Kristina Johansson,Semil P. Choksi,Dan Cheng,Leqian Liu,Dingyuan I Sun,Lorna Zlock,Walter L. Eckalbar,Walter E. Finkbeiner,David J. Erle
标识
DOI:10.1165/rcmb.2020-0149ma
摘要
The human airway epithelium is essential in homeostasis, and epithelial dysfunction contributes to chronic airway disease. Development of flow-cytometric methods to characterize subsets of airway epithelial cells will enable further dissection of airway epithelial biology. Leveraging single-cell RNA-sequencing data in combination with known cell type-specific markers, we developed panels of antibodies to characterize and isolate the major airway epithelial subsets (basal, ciliated, and secretory cells) from human bronchial epithelial-cell cultures. We also identified molecularly distinct subpopulations of secretory cells and demonstrated cell subset-specific expression of low-abundance transcripts and microRNAs that are challenging to analyze with current single-cell RNA-sequencing methods. These new tools will be valuable for analyzing and separating airway epithelial subsets and interrogating airway epithelial biology.
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