PLGA公司
材料科学
纳米颗粒
蒸馏水
扫描电子显微镜
化学工程
聚合物
核化学
纳米技术
复合材料
化学
色谱法
工程类
作者
Huihao Qin,Wei Zhang,Yi Luo,Yubo Wei,Shenglu Sun,Jingya Chen
出处
期刊:Science of Advanced Materials
[American Scientific Publishers]
日期:2019-12-01
卷期号:11 (12): 1816-1824
被引量:7
标识
DOI:10.1166/sam.2019.3723
摘要
Polylactic acid glycolic acid (PLGA), a polymer material, is used as a shell and perfluorooctane (PFOB) as the core. Fe 3 O 4 @SiO 2 nanoparticles are distributed in the shell to prepare a magnetic phase change multifunctional nano emulsion contrast agent (Fe 3 O 4 @SiO 2 /PLGA/PFOB). The ultrasound, magnetic resonance imaging and CT imaging effects of the contrast agent on normal rat liver parenchyma are investigated. Methods: Single emulsification method is used to prepare Fe 3 O 4 @SiO 2 -loaded polymer Fe 3 O 4 @SiO 2 /PLGA/PFOB nanoparticles encapsulated with PLGA. In addition, polymer Fe 3 O 4 @SiO 2 /PLGA nanoparticles loaded with Fe 3 O 4 @SiO 2 nanoparticles, PLGA@PFOB nanoparticles coated with PLGA and blank PLGA nanoparticles are prepared as experimental control group. Its surface morphology, internal structure, particle size, potential and other general characteristics are tested. RAW264.7 mouse mononuclear macrophages are cultured in vitro . Prussian blue staining is used to observe macrophage phagocytosis after Fe 3 O 4 @SiO 2 /PLGA/PFOB contrast agent is added to incubate for 3 hours. MTT method is used to detect the effect of contrast agent on cell activity. The macrophage suspension engulfed with Fe 3 O 4 -PFOB is examined by ultrasound, magnetic resonance and CT. The results showed that Fe 3 O 4 @SiO 2 /PLGA/PFOB is brown emulsion after being dissolved in double distilled water. Under the light microscope and scanning electron microscope, Fe 3 O 4 @SiO 2 /PLGA/PFOB is spherical, with regular shape, uniform size, good dispersity and smooth surface. Macrophages are dependent on the phagocytosis of the contrast medium without affecting the activity of macrophages. Moreover, in vitro imaging experiments, compared with the control group, macrophages phagocytized by Fe 3 O 4 @SiO 2 /PLGA/PFOB have obvious enhancement effects in ultrasound, magnetic resonance and CT imaging. In conclusion, Fe 3 O 4 @SiO 2 /PLGA/PFOB has good stability in vivo , can be engulfed by liver macrophages, and can enhance ultrasound, magnetic resonance and CT imaging of liver parenchyma. It is a safe and effective multifunctional ultrasound contrast agent.
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