[Establishment of Secondary HLH Mouse Model and Effect of Ruxolitinib on Disease Manifestations of Model Mide].

To establish a secondary hemophagocytic lymphohistiocytosis(HLH) mouse model, and to investigate the effect of ruxolitinib on the disease manifestation of model mice.Wild type C57BL/6 mice were randomly divided into 4 groups: two groups of mice were intraperitoneally injected with CpG oligodeoxynucleotide 1826 (CpG-ODN1826) every other day to induce HLH, and other two groups were control groups. One group of the CpG-ODN1826 groups and one of the control groups were given ruxolitinib, and other two groups were given the same amount of PBS. Blood samples, serum ferritin and hepatic/spleen weights of experimental mice were detected and serum cytokine levels were measured by ELISA.Compared with the control groups, the levels of white blood cells, hemoglobin and platelets in the CpG-ODN1826 groups were significantly lower (P＜0.05); and liver/body weight, spleen/body weight, serum ferritin, sCD25, IL-10, IL-1β, IFN-Ƴ, IL-12p70, GM-CSF, TNF-α and IL-18 levels significantly increased (P＜0.05). There was no significant difference in the levels of IL-2, IL-4, IL-5, IL-6, IL-22, IL-13, IL-27 and IL-23 between the two groups (P＞0.05). The spleen in CpG group had disordered internal structure, expanding red pulp and hyperplastic nucleated cells. The liver had severe perivascular inflammations. The spleen/weight of the ruxolitinib-treated mice in the CpG-ODN1826 group was significantly smaller than that of the unapplied ruxolitinib (P＜0.05).The CpG-ODN1826 can induce secondary HLH symptoms in wild type C57BL/6 mice. Ruxolitinib can alleviate the symptoms of splenomegaly in HLH model mice.继发性HLH小鼠模型的建立及芦可替尼对模型小鼠疾病表现的影响.建立一种继发性噬血细胞性淋巴组织细胞增生症（HLH）小鼠模型，观察芦可替尼对模型小鼠疾病表现的影响。方法：。结果：。结论：。.将野生型C57BL/6小鼠随机分为4组，其中2组小鼠给予隔天腹腔注射CpG寡脱氧核苷酸1826（CpG-ODN1826）诱导产生HLH，另2组为对照组。CpG-ODN1826组和对照组中各有1组给予芦可替尼，其余2组给予等量PBS。检测实验小鼠血常规指标、血清铁蛋白和肝脾重量，用ELISA方法检测血清细胞因子水平.CpG-ODN1826组小鼠与正常对照组相比，白细胞数、血红蛋白水平、血小板数量明显降低（P＜0.05）；肝/体重、脾/体重、血清铁蛋白水平、sCD25、IL-10、IL-1 β、IFN-Ƴ、IL-12p70、GM-CSF、TNF-α和IL-18表达水平均明显升高（P＜0.05）；而IL-2、IL-4、IL-5、IL-6、IL-22、IL-13、IL-27、IL-23表达水平均无明显差异（P＞0.05）。CpG-ODN1826组小鼠脾组织内部结构排列紊乱，红髓部分明显扩大，有核细胞大量增生；肝组织血管周围有大量炎症细胞聚集。在CpG-ODN1826组中应用芦可替尼的小鼠脾/体重较未应用芦可替尼显著缩小（P＜0.05）.CpG-ODN1826可以诱导野生型C57BL/6小鼠产生继发HLH反应，建立继发HLH模型，而芦可替尼可以减轻HLH模型小鼠脾大症状.