The activity of Ofloxacin and Ciprofloxacin against 50 strains of M. tuberculosis were investigated in-vitro on Löwenstein-Jensen medium. Minimal Inhibitory Concentration (MIC90) of Ofloxacin and Ciprofloxacin were found 1 mg/L and 4 mg/L, respectively. There were no difference in susceptibility to Ofloxacin and Ciprofloxacin between strains which were susceptible or resistant to Standard Anti-tubercular drugs.