Identification and Control Efficacy of Bacillus amyloliquefaciens Strain SDF-005 against Plant Anthracnose

Through morphological characteristics observation,physiological and biochemical characteristics measurement,as well as 16S rDNA and gyrB gene sequence determination,we ascertained the taxonomic status of an antagonistic bacterial strain SDF- 005 isolated from the soil of walnut orchard,and tested the inhibitory activity of this strain to 5 plant anthracnose diseases and its control efficacy against walnut anthracnose in orchard. Strain SDF- 005 was identified as Bacillus amyloliquefaciens. This strain had stronger inhibitive effect on the mycelial growth of 5 plant anthracnose diseases( especially grape anthracnose disease),the inhibition zone diameter ranged from 12. 00 mm to 19. 33 mm,and the inhibition rate reached 75. 00% ~ 83. 06%. The inhibitive effect of SDF- 005 against the pathogens of plant anthracnose was shown mainly by its effect on mycelia. The various metabolites of SDF- 005 destroyed the structure of mycelial wall,and caused the expansion,distortion,warping and disintegration of mycelia. Using the 400 ~ 600- times dilution of SDF- 005 WP to control walnut anthracnose in the forest,the control efficacy was above 75%.