[Effects of 6 kinds of metal elements on meiotic maturation and in vitro fertilization on mouse oocyte].

To study the effects of Cd, Pb, Ni, Mn, Cr and Al on reproductive toxicity, the meiotic maturation and in vitro fertilization(IVF) of mouse oocytes were studied using in vitro culture and IVF of mouse oocytes. The results showed that except Al(NO3)2, the other groups [CdCl2, Pb(NO3)2, NiCl2, MnSO4 and CrCl3] could significantly reduce the number of superovulation oocyte. All of them could inhibit the meiotic maturation, the rates of IVF and extruding of the first polar body, but there was no impact on the germinal vesicle breakdown (GVBD) in vivo. The results also showed that except Al(NO3)3, other elements could obviously inhibit germinal vesicle breakdown of mouse oocytes. The results suggested that these elements might damage the process of meiotic maturation of oocyte of women at child-bearing age oocyte and reduce the fertility and reproductive capacity and result in sterile.