Simultaneous Quantitative Analysis of Three Flavonoids in Pericarpium Citri Reticulatae by Multi-components Assay by Single Marker

Objective: To establish a method for determination of hesperidin,nobiletin and tangeretin in Pericarpium Citri Reticulatae. And to verify the accuracy and feasibility of the method. Method: Using hesperidin as standard substance,the relative correction factor of the other two flavonoids were determined by HPLC. Using the external standard method and the relative correction factor calculated the content of the three flavonoids,then comparing the results. Result: The results of external standard method and quantitative analysis of multicomponents by one marker( QAMS) have no significant difference. Conclusion: Determination of hesperidin, nobiletin and tangeretin by QAMS is a accurate and feasible method.