Separation and Structural Analysis of Polysaccharides BBP3-1 from Blueberry

In this study the raw polysaccharide was obtained from Blueberry by hot water extraction and ethanol precipitation method coupled with pectinase optimum.Then it was discolored by H2O2,enzymatic hydrolyzed and then removed of protein by combining trichloroacetic acid and n-butanol.Polysaccharide(BBP3-1) was purified using DEAE Sepharose Fast Flow ion exchange echromatography and Sephacryl S-300 HR gel filtration chromatography(GFC).UV spectra measurements confirmed there was no protein and nucleic acid.HPLC analysis showed that the average molecular weight(Mw) was 18643Da,the numerical average weight(Mn) was 5851 Da,the peak molecular weight(Mp) was 3554 Da, the width of molecular weight(Mw/Mn) was 3.186.GC analysis showed that the polysccharide BBP3-1 was composed of GalUA,Xyl and Glu with a molar proportion of 1∶1.5∶2.The IR and NMR spectrum analysis indicated the chain of the polysccharide BBP3-1 was dominant by 1,6-glucopyranose chain.It was an acidic β polysaccharide containing GalUA,Xyl,Glu and Rha but without Man.