Expression of osteopontin in colon cancer tissue and detection of osteopontin in serum

Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P0.01).The OPN mRNA expression and the serum level in patients with recurrence one year after operation were higher than those without recurrence(P0.01).Conclusion Osteopontin is one of the tumor markers and it could be used in diagnosis and prognosis evaluation of colon cancer.