VBAI is used to establish intelligent biomedical image processing and analysis program to solve the problems existed in the present biomedical image processing and analysis software. Three applications about microsphere imaging,red-blood cell imaging and pollen imaging were performed as examples of application. The results indicate that VBAI can create image processing and analysis program easily for particular situations and purposes. It can give objective result by detecting mass of images automatically and quickly. VBAI is a powerful tool to establish biomedical image processing and analysis program for laboratories with different purposes.