Identification and sequence analysis of a pseudorabies virus isolated from sheep

To diagnose a suspected sheep pseudorabies case and analyse the sequence of gC gene of sheep pseudorabies virus(PRV),the brain of sheep with clinical symptom of sheep pseudorabies from a farm in Shandong Province was collected in 2012. PRV in the brain tissue was confirmed by observation under electron microscope and PCR amplification and sequence analysis of the gC gene.Phylogenetic analysis was made based on the gC gene.The results confirmed a PRV case,in which the isolate was named as China/SD/2012.Phylogenetic analysis demonstrated that there was a close genetic relationship between the PRV isolate and other PRVs which isolated from Argentina pigs in 1998.This study provided an useful information for the molecular epidemiology of sheep PRV in China.