Objective To investigate and analyze the prophylactic usage of antibiotics in perioperative period in our hospital so as to provide help for using antibiotics rationally.Methods Totally 300 cases from January to June in 2008 which were selected randomly were retrospective investigated and analysed.Results Among 300 cases,antibiotics was used in 292 cases(97.3%),cephalosporins took the lead on the list of the most frequent used antibacterial drugs;There are 242 cases(82.9%) which used rationally before and during operation,and 182 cases(62.3%) which used rationally after operation.Conclusions During perioperative period,the prophylactic usage of antibiotics is basically rational in our hospital.But the high class antibiotics used in perioperative period;the timing and duration of prophylactic use of drugs tends to be on the long side and exceed the duration of antibiotics instruction principle in some cases.Therefore,intervention is urgently needed to tackle these problems.