Involvement of PKC in transient outward potassium current(I_A) inhibited by N/OFQ in acutely dissociated rat cerebral parietal cortical neurons

Objective To study the involvement of protein kinase C(PKC) in transient outward potassium current(IA) inhibited by N/OFQ in acutely dissociated rat cerebral parietal cortical neurons.Methods Whole-cell voltage clamp technique was used to observe the effect of chelerythrine,a inhibitor of PKC,on the inhibition IA by Orphanin FQ/nociceptin(N/OFQ).Results ①Bath applications of N/OFQ resulted in bidirectional modulation of IA in different potentials in presence of chelerythrine(n=10,P0.05).②The amplitude of IA was attenuated by N/OFQ at +60 mV in presence of chelerythrine(n=10,P0.05,compared with control).N/OFQ's percent inhibition on IA in presence of chelerythrine was(33.9±6.6)% that was more significantly than that in the control test without chelerythrine,(23.20±2.17)%.Conclusion N/OFQ likely modulates IA in a voltage-dependent manner in presence of chelerythrine in acutely dissociated rat cerebral parietal cortical neurons.