The Effect of Proliferation and Apoptosis of Human Non-small Cell Lung Cancer A549 by Gemcitabine in Vitro

Objective To observe the effect of proliferation and apoptosis of human non-small cell lung cancer treated by Gemcitabine. Methods The apoptosis of A549 induced by Gemcitabine were detected by Annexin V-PI double-labeling, flow cytometry, TUNEL approach. The changes of the expression of bcl-2 after Gemcitabine treatment was examined by Immunohistochemistry. Results The inhibitory action of cell growth was seen in A549 cell dealing with Gemcitabine. It could also promote the occurrence of apoptosis and induce the S-phase arrest. The apoptosis induced by Gemcitabine was accompanied with the down-regulation of bcl-2. Conclusion Gemcitabine could inhibit the cell proliferation and induce the occurrence of apoptosis of A549 cell. The induced apoptosis, which had certain relationship between the expression of bcl-2, was one of the mechanisms of anti-tumor actions.