Sequence Analysis of the Major Coding Sequence of E2 Gene of Different Generations of Hog Cholera Field Virus

The major coding sequence of E2 of different generations of HCV field strains were analysed by reverse trancriptase polymerse chain reation (RT PCR) and sequenced.The results showed that the nucleotide sequence and amino acid sequence of the major coding sequence of E2 gene of differents generation of every field strain were conservative. The nucleotide sequence and amino acid sequence of all generation of every strain were not change.The identity of nucleotide sequence and amino acid sequence were 100%.Compared with the 02 strain,the identity of nucleotide sequence and amino acid sequence of the others was 82.0%～99.5%,84.1%～98.6%. The phylogenetic tree was divided into 2 groups,per group was divided into 2 subgroups.03,05 and 22 strains were distributed in 1 group.02 and 06 strains were divided into the 2 subgroup.Different generations of every strain were all divided into the same group,sama subgroup. It confirmed initinally that molecular structure of hog cholera virus was genetically stable.