Sesamin represses protein expression of nitrotyrosine,NF-κB and matrix metalloproteinase-9 in myocardium of metabolic syndrome rats

Aim To investigate the possible in vivo protective effects of sesamin on myocardial remodeling in metabolic syndrome(MS) rats.Methods MS rat model was induced by operation of two-kidneys with one-clip and fed with a high-fat,high-sucrose diet.sesamin(120,60 and 30 mg·kg-1·d-1) was orally administered in MS rats.Eight weeks later,rats were sacrificed,heart wet weight(HWW) and left ventricle wet weight(LVWW) were recorded,total-antioxidative activity(T-AOC) in serum was measured,and protein expression of nitrotyrosine(NT),NF-κB and matrix metalloproteinase-9(MMP-9) in myocardium was detected by Western blot.In addition,pathological changes in myocardium were observed with HE and Masson staining and collagen volume fraction(CVF) was measured.Results Compared with MS rats,treatment with 120 and 60 mg·kg-1 sesamin decreased HWW,enhanced T-AOC,and reduced NT,NF-κB and MMP-9 protein expression.Treatment with 120 mg·kg-1 sesamin decreased LVWW and CVF,and obviously ameliorated myocardial pathologic lesions.Conclusion Sesamin improves myocardial remodeling through reduction of NF-κB and MMP-9 expression in addition to alleviation of oxidative stress in MS rats.