Preparation of homoharringtonine imprinted polymer by precipitation polymerization

The precipitation polymerization method was optimized by using a L9(34) orthogonal factorial design for the preparation of homoharringtonine imprinted polymer (HIP). Optimization of the synthesis condition for the HIP was performed by investigating the influence of four factors,i.e. solvent (acetonitrile) dosage (A),reaction temperature (B),functional monomer (methacrylic acid,MAA) dosage (C) and crosslinker (ethylene glycol dimethyl acrylate,EGDMA) dosage (D),and the interaction of such factors on the adsorption capacity of HIP obtained under different conditions to the template molecule. The results indicated that the influence of these factors on the adsorption capacity of the HIP decreased in the order of C,D,A and B and the optimized synthesis condition was as follows:0.12 mmol MAA,0.4 mmol EGDMA,2.5 mL acetonitrile,reaction temperature at 65 ℃ under the fixed template dosage (0.01 mmol). Also,the interaction of such factors could influence the adsorption capacity of HIP to the template.