Breeding and Screening of a High-Yield Tannase-Producing Strain

Tannase plays an important role in the food and fine chemical industries.The breeding and screening of a strain with high tannase activity are desired.A tannase-producing strain,DNM1-39,was isolated from resources rich in tannin.The activity of tannase in solid state medium after fermentation by the strain was 1.58 U/g.The strain was identified as Aspergillus through morphologic observation.A mutant strain named as UD-6 was obtained from DNM1-39 mutagenization with ultraviolet irradiation alone,DES treatment alone and both of them.The tannase activity produced by UD-6 was 2.80 U/g.The tannase-producing ability of UD-6 remained the same after continuous passages and displayed excellent genetic stability.