In order to explore dissolution characteristics of calcium in the shell of Penaeus vannamei in different organic acid systems,the influence of different conditions such as different organic acid systems,dissolution time,dissolution means,dissolution temperature and glycine in dissolving rates of calcium were discussed by experiments.The results showed that the content of total calcium in the shell of Penaeus vannamei was 31.5%(dry weight).According to the results of dissolution in different organic acid systems,the order was citric acid acetic acid tartaric acid.In citric acid systems,the dissolving rate was the highest when time was 20 min and temperature was 80 ℃ in water bath.Adding glycine can greatly improve the dissolving rate of calcium.