生物分子
荧光相关光谱
荧光
生物系统
光谱学
纳米技术
分子
高分子
生物物理学
物理
化学
材料科学
生物
光学
生物化学
量子力学
作者
Miguel Ángel Medina,Petra Schwille
出处
期刊:BioEssays
[Wiley]
日期:2002-07-23
卷期号:24 (8): 758-764
被引量:162
摘要
The recent development of single molecule detection techniques has opened new horizons for the study of individual macromolecules under physiological conditions. Conformational subpopulations, internal dynamics and activity of single biomolecules, parameters that have so far been hidden in large ensemble averages, are now being unveiled. Herein, we review a particular attractive solution-based single molecule technique, fluorescence correlation spectroscopy (FCS). This time-averaging fluctuation analysis which is usually performed in Confocal setups combines maximum sensitivity with high statistical confidence. FCS has proven to be a very versatile and powerful tool for detection and temporal investigation of biomolecules at ultralow concentrations on surfaces, in solution, and in living cells. The introduction of dual-color cross-correlation and two-photon excitation in FCS experiments is currently increasing the number of promising applications of FCS to biological research.
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