Isolation of Japanese encephalitis and Getah viruses by Aedes albopictus clone C6/36 cells and by suckling mouse brain inoculation in Nagasaki, 1980.

Abstract: Virus isolation was performed from field-caught female Culex tritaeniorhynchus and from postmortem brain materials of an encephalitis patient by inoculation into Aedes albopictus clone C6/36 cells and into suckling mouse brains (SMB). From 18,000 mos-quitoes in 180 pools, Japanese encephalitis (JE) virus was isolated from 17 pools by C6/36 cells, in contrast to 8 pools by SMB. Seven pools with positive JE virus isolation by SMB were also with positive results by C6/36 cells. There was a single pool with positive JE virus isolation by SMB with negative result by C6/36 cells. Inoculation into SMB could not detect JE virus from 10 mosquito pools and postmortem brain materials which yielded JE virus by C6/36 cells. Getah (GET) virus was detected from 5 mosquito pools by C6/36 cells, and 2 out of the 5 pools also yielded GET virus by SMB. A single pool which produced JE and GET viruses by C6/36 cells produced only JE virus by SMB. No significant difference was observed between the virus strains isolated from mosquito pools which yielded the virus by both methods and the virus strains isolated only by C6/36 cells, in terms of neutralization test and of plating efficiency on C6/36 cells at