荧光
共域化
溶酶体
荧光寿命成像显微镜
双光子激发显微术
荧光显微镜
生物物理学
显微镜
生命科学中的荧光
化学
过氧化氢
费斯特共振能量转移
细胞生物学
生物
生物化学
病理
光学
酶
医学
物理
作者
Mingguang Ren,Beibei Deng,Jianyong Wang,Xiuqi Kong,Zhan-Rong Liu,Kai Zhou,Longwei He,Weiying Lin
标识
DOI:10.1016/j.bios.2015.12.046
摘要
Hydrogen peroxide (H2O2) plays a crucial role in many biological processes in the human body. As our understanding of the complexity of physiological H2O2 in lysosome, investigative tools are required to make sense of this interconnectivity. Toward this goal, we have developed a new example of a fast responsive and lysosome-targeted two-photon H2O2 fluorescent probe (Lyso-HP) with a large turn-on fluorescence signal (80-fold fluorescence enhancement). The addition of H2O2 to Lyso-HP results a dramatic fluorescence enhancement around 550 nm. The probe could image exogenous and endogenous H2O2 in living cells and the probe was located in lysosomes with high colocalization coefficient (0.96) compared with LysoTracker. The large fluorescence enhancement of the two-photon probe Lyso-HP renders it attractive for imaging H2O2 in living tissues with deep tissue penetration. Significantly, the probe is feasible for fluorescently monitoring H2O2 level changes in lysosomes and suitable for fluorescence imaging of H2O2 in living tissues with deep penetration by using two-photon microscopy.
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