s Background Whipped cream, an oil-in-water emulsion derived from separating skim milk from whole milk, typically contains 30-38% fat and is widely used in various food applications. The complex chemical composition and structural characteristics of milk fat, combined with diverse formulations and processing technologies, play a pivotal role in determining the stability and whipping properties of whipped cream. Scope and approach This review offers an integrative perspective that systematically elucidates the multifaceted roles of fatty acid composition, triglyceride molecular configuration, crystallization kinetics, and partial coalescence of milk fat in shaping the structural and functional properties of whipped cream. It highlights emerging analytical techniques such as differential scanning calorimetry, confocal laser scanning microscopy, and advanced chromatographic methods. Moreover, the review proposes a novel framework for evaluating the synergistic interactions between milk fat and whipped cream structure, aiming to support the rational design of nutritionally enhanced and functionally stable whipped cream products. The incorporation of health-oriented lipid components, along with discussions on their bioavailability and metabolic impacts, further broadens the scope and relevance of this review. Key findings and conclusions The composition and structure of milk fat, along with processing techniques, are key to regulating whipped cream stability and foaming. This paper reviews how understanding milk fat composition and structure affects product storage stability, microstructure and functionality. Furthermore, it highlights key challenges and areas requiring further research, which are essential for enhancing the functionality and expanding the use of whipped cream in the food industry.