A new isoflavone glycoside from the stems of Eclipta prostrata (L.) L. with glycosidase inhibitory activity

A new isoflavone glycoside, prostratanoside A (1), along with four known compounds (2-5), was isolated from the stems of Eclipta prostrata (L.) L. Their structures were elucidated through comprehensive spectroscopic (NMR and MS) and physicochemical analyses. All isolated compounds were evaluated for their in vitro α-glucosidase inhibitory activity. Notably, compound 1 displayed the most potent inhibition, with an IC₅₀ value of 10.3 ± 1.2 μM. Besides, the potential binding sites of 1 were predicted by molecular docking.