一致性
呼吸系统
病毒
病毒学
生物
计算生物学
频道(广播)
化学
肺病毒科
计算机科学
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019年冠状病毒病(COVID-19)
分子生物学
临床诊断
物理
医学
作者
Yanan Lin,Dawei Jiang,Xingpeng Dong,Yilin Li,Xiao Yu Wu,Ruoxuan Li,Li Feng,Dan Sun,Yanyan Yu
摘要
A label-free, orthogonal dual-channel CRISPR-Cas platform is developed for the simultaneous detection of diverse pathogens. Cas12a is reported by a G4-PPIX complex, and Cas13 by a split Broccoli-DFHBI aptamer, which ensure exclusive channel specificity and eliminate optical cross-talk. Clinical tests on 50 samples show 100% concordance with PCR.
科研通智能强力驱动
Strongly Powered by AbleSci AI