Bridges are crucial components of transportation networks, however, their structural performance deteriorates over time due to increasing traffic loads, material deterioration, and natural disasters. Rapid assessment of the load-carrying capacity of highway bridges is important for maintenance planning and rescue route management after natural disasters. Conventional load-test-based performance evaluation methods are labor-intensive, expensive, and potentially hazardous. To overcome these challenges, this paper proposes a rapid load-carrying capacity evaluation method for highway bridges using impact testing data and virtual load testing. The contributions of this study include: (1) the development of an automated bridge flexibility identification method that employs impact testing data and a multi-scale peak extraction algorithm; (2) the development of a multi-point deflection prediction and load-carrying capacity assessment method using identified modal flexibility and virtual load testing. Validation through experimental validation of a simply-supported T-shaped beam bridge and field testing on a three-span continuous concrete box-girder bridge confirms the effectiveness and robustness of the developed method. This approach significantly simplifies the load-carrying capacity assessment process, offering a practical solution for the rapid evaluation of numerous highway bridges.