CuO2-assisting-Zn single atom hybrid nanozymes for biofilm-infected wound healing

Single-atom catalysts (SACs), characterized by atomically dispersed single metal atoms, provide superior antibacterial properties over common nanozyme materials due to their satisfactory catalytic activity. However, when used for the treatment of biofilm-infected wounds, SACs are still unable to effectively solve severe microbial infection due to the insufficient supply of reactive oxygen species (ROS). Herein, a new hybrid nanozymes consisting of Zn single-atom catalysts and copper peroxide (Zn SACs@CuO2) were prepared to realize the upgrading of ROS generation and the combination of photothermal/chemodynamic (PTT/CDT) therapy for biofilm-infected wound. Specifically, CuO2 exhibited not only excellent photothermal property, but also H2O2-producing and Fenton-like catalytic activities, which assisted Zn SACs to accelerate the ·OH conversion of endogenous and exogenous H2O2. Compared with single antimicrobial strategies of Zn SACs, Zn SACs@CuO2 hybrid nanozymes exhibited stronger therapeutic effects of biofilm destruction, immune activation and inflammation improvement. In conclusion, this work developed a hybrid single atom nanozyme material with for efficient biofilm-infected wound healing.